6 Oct 2020 Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA- insensitive phe- notypes of coi1 mutants and JAZ-DJas (JAZ1, JAZ5, and

Direct targets of ABI4 and ABI5 regulation. To emphasize the effects of ABI4 and ABI5 expression, we analyzed 11 day old seedlings, a developmental stage when seed specific transcription factors are not normally expressed but overexpression of ABI4 or ABI5 enhances ABA-inducible responses (Brocard et al. 2002; Söderman et al. 2000).

Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5. Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes. 2020-03-02 · Consistently, PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark. Moreover, we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9, and that this interaction is not regulated by ABA. Overexpression of ABI5 results in the plants being hypersensitive to abscisic acid such that they respond to low levels of abscisic acid which have no effect on wild type plants. One aspect of the invention is seeds, seedlings or plants which are resistant to drought because they overexpress ABI5.

Abi5 overexpression

(A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ Moreover, overexpression of ABI5 (ABI5-OE-8) in the JAZ8-ΔJas ABI3-OE background rendered the transgenic lines much more sensitive to ABA and COR during seed germination . Similar data were also obtained when ABI3 and ABI5 were expressed in the JAZ5-ΔJas background ( Supplemental Figures 12C and 12D ). Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions.

Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes.

ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days.

2020-04-11 · Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots.

ABI5 overexpression rescued the growth arrest phenotype at low CO 2.

One aspect of the invention is seeds, seedlings or plants which are resistant to drought because they overexpress ABI5. ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days.
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ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought.

Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. ABI5 overexpression rescued the growth arrest phenotype at low CO 2.
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Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas …

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ABI5 in the hy5 mutant background restored its ABA sensitivity and overexpression of ABI5 in the wild-type background-enhanced light response. These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5. Results HY5 Mediates ABA Response in Roots and Is Required for Osmotic

Together, our results reveal a previously uncharacterized signaling module in which JAZ Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions.